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In brief, serum proteins were alkylated with iodoacetamide, digested with trypsin and spiked with heavy isotope-labelled synthetic peptide analogues (PEPotec, Thermo Fisher Scientific, USA)

Modulates nitric oxide pathways, improving blood flow and nutrient delivery

Secondly, the identification of specific molecular targets and pathways through which AS-IV exerts its effects could facilitate the design of targeted clinical trials, allowing for a more precise understanding of its mechanisms of action

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Coffee polyphenols can change genes involved in glutathione synthesis